Biochemical Assay Services
Quantitative and Qualitative Biochemical Assay Services for Target Identification & Validation, Hit Identification, Hit-to-Lead, Lead Optimization, and Clinical Candidate Selection
PozeSCAF provides quantitative biochemical assay services that generate reliable, decision-ready data to support target identification and validation, hit identification, hit-to-lead, lead optimization, and clinical candidate selection. Our scientists develop and execute fit-for-purpose biochemical assays tailored to each biological target, enabling informed decision-making throughout early drug discovery.
Biochemical Assay Expertise
PozeSCAF provides quantitative biochemical assay services to characterize target activity, evaluate compound potency, measure binding affinity, investigate mechanism of action, assess selectivity, and support enzyme kinetics throughout early drug discovery. Our scientists develop, optimize, and execute fit-for-purpose assays tailored to each biological target and research objective, generating reliable data that supports target validation, hit identification, hit-to-lead, and lead optimization.
Comprehensive Biochemical Assay Capabilities
PozeSCAF offers a comprehensive portfolio of biochemical assays supporting target characterization, compound evaluation, and early drug discovery. Our services span enzyme activity, target binding, potency determination, mechanism of action, selectivity profiling, and custom assay development, providing quantitative data to guide informed discovery decisions.
Enzyme Activity & Inhibition Assays
PozeSCAF develops quantitative enzyme activity and inhibition assays to evaluate target function, characterize inhibitor activity, determine concentration-dependent responses, and assess compound efficacy. Assays are optimized for target biology and project objectives to support target validation, hit confirmation, SAR studies, and lead optimization with reliable experimental data.
Applications
- Enzyme activity characterization
- Enzyme inhibition studies
- Hit identification and confirmation
- Compound potency evaluation
- Target validation
- Comparative profiling of candidate molecules
- Lead optimization
Target Binding & Affinity Assays
Our target binding assays characterize molecular interactions, binding affinity, target engagement, and interaction kinetics to validate screening hits and prioritize lead compounds. Assays are tailored to the biological target and provide quantitative data supporting medicinal chemistry optimization and candidate selection.
Applications
- Target engagement studies
- Binding affinity determination
- Hit confirmation and validation
- Compound ranking and prioritization
- Lead optimization
- Target specificity assessment
- Candidate selection
Potency & Concentration–Response Assays
PozeSCAF performs quantitative potency and concentration–response assays to determine IC₅₀, EC₅₀, and dose–response relationships for compound evaluation. These assays provide critical data for hit confirmation, SAR analysis, lead optimization, and compound prioritization throughout early drug discovery.
Applications
- Compound potency determination
- IC₅₀ and EC₅₀ determination
- Dose–response analysis
- Hit confirmation and prioritization
- Lead optimization
- Comparative evaluation of candidate molecules
- Medicinal chemistry decision support
Mechanism of Action (MoA) Studies
Mechanism of action studies investigate how compounds modulate biological targets through inhibition mechanisms, target engagement, substrate interactions, and enzyme kinetics. These studies generate mechanistic insights that support lead optimization, candidate differentiation, and informed decision-making during drug discovery.
Applications
- Mechanism of action characterization
- Target engagement confirmation
- Inhibition mechanism studies
- Competitive and non-competitive inhibition analysis
- Substrate and cofactor interaction studies
- Lead optimization
- Candidate differentiation and prioritization
- Therapeutic hypothesis validation
Selectivity Profiling Assays
PozeSCAF performs selectivity profiling to evaluate compound activity across related targets and biologically relevant off-targets. These studies help identify selective lead molecules, guide medicinal chemistry refinement, reduce development risk, and support candidate selection.
Applications
- Target selectivity assessment
- Off-target activity evaluation
- Lead optimization
- Candidate prioritization
- Comparative profiling of lead compounds
- Medicinal chemistry decision support
- Early risk assessment
Custom Biochemical Assay Development
Every discovery program presents unique scientific challenges. PozeSCAF develops customized biochemical assays tailored to specific targets, pathways, and research objectives. From assay design and optimization to validation and technology transfer, we establish fit-for-purpose workflows that support screening, hit validation, lead optimization, and advanced biochemical characterization.
Capabilities
- Custom biochemical assay design and development
- Assay optimization and validation
- Assay transfer and technology transfer
- Method adaptation and refinement
- Assay troubleshooting and performance improvement
- Optimization of assay conditions and detection parameters
- Development of target-specific biochemical assays
- Fit-for-purpose assay workflows for early drug discovery
Applications
- Novel target evaluation
- Challenging enzyme systems
- Hit validation
- Lead optimization
- Assay development for emerging therapeutic targets
- Client-specific research programs
- Platform establishment for screening campaigns
- Biochemical characterization of new molecular entities
Supported Detection Technologies
- Fluorescence-based detection
- Luminescence assays
- TR-FRET / HTRF
- Fluorescence Polarization (FP)
- Colorimetric assays
- UV-Visible spectroscopy
- AlphaScreen® / AlphaLISA® (where applicable)
- Microplate reader-based detection
Assay Technologies
Fluorescence-Based Assays
Sensitive fluorescence detection enables quantitative measurement of enzyme activity, molecular interactions, and compound responses with excellent assay performance across multiple biochemical applications.
Time-Resolved Fluorescence Technologies (TR-FRET / HTRF)
Homogeneous TR-FRET and HTRF assay formats provide highly sensitive detection with low background interference, making them ideal for target engagement, enzyme activity, binding studies, and high-throughput biochemical screening.
Fluorescence Polarization (FP)
Fluorescence polarization assays enable quantitative analysis of molecular binding events, ligand displacement, and biomolecular interactions without requiring separation steps, supporting affinity determination and compound screening.
Luminescence Assays
Luminescence-based detection provides exceptional sensitivity and broad dynamic range for enzyme activity measurements, reporter assays, ATP-based detection, and quantitative biochemical analysis.
Absorbance & Colorimetric Assays
Colorimetric and absorbance-based assays offer reliable quantitative analysis of enzyme activity and biochemical reactions using well-established assay principles suitable for routine characterization and validation studies.
UV-Visible Spectroscopy
UV-Visible detection supports quantitative biochemical measurements including enzyme kinetics, substrate conversion, reaction monitoring, and absorbance-based analytical applications.
AlphaScreen® & AlphaLISA®
AlphaScreen® and AlphaLISA® technologies enable highly sensitive no-wash detection of biomolecular interactions, ligand binding, and protein-based assays, making them well suited for complex biochemical studies and screening applications.
ADP-Glo™ Kinase Assays
ADP-Glo™ technology enables sensitive quantification of kinase activity through ADP detection, supporting kinase inhibitor discovery, potency determination, and enzymatic characterization.
LanthaScreen®
LanthaScreen® assay technology supports high-sensitivity biochemical and kinase assays for quantitative evaluation of compound activity, target engagement, and pharmacological characterization.
Assay Quant Technologies (AQT):
Through Assay Quant Technologies’ patented PhosphoSens® platform, we provide real-time fluorescence-based kinase and phosphatase activity assays for inhibitor profiling and enzyme kinetics.
Ready to Advance Your Drug Discovery Program?
Whether you need standard biochemical assays, custom assay development, target characterization, potency evaluation, or mechanism of action studies, PozeSCAF’s scientists are ready to generate quantitative biochemical data that supports confident decision-making throughout early drug discovery.
Discuss your project with our scientific team to identify the right biochemical assay strategy for your research program.